本文整理了Java中org.broadinstitute.gatk.utils.sam.GATKSAMRecord.getInferredInsertSize()
方法的一些代码示例,展示了GATKSAMRecord.getInferredInsertSize()
的具体用法。这些代码示例主要来源于Github
/Stackoverflow
/Maven
等平台,是从一些精选项目中提取出来的代码,具有较强的参考意义,能在一定程度帮忙到你。GATKSAMRecord.getInferredInsertSize()
方法的具体详情如下:
包路径:org.broadinstitute.gatk.utils.sam.GATKSAMRecord
类名称:GATKSAMRecord
方法名:getInferredInsertSize
暂无
代码示例来源:origin: broadgsa/gatk-protected
@Override
protected Double getElementForRead(final GATKSAMRecord read, final int refLoc) {
return (double)Math.abs(read.getInferredInsertSize());
}
}
代码示例来源:origin: broadgsa/gatk
/**
* is this base inside the adaptor of the read?
*
* There are two cases to treat here:
*
* 1) Read is in the negative strand => Adaptor boundary is on the left tail
* 2) Read is in the positive strand => Adaptor boundary is on the right tail
*
* Note: We return false to all reads that are UNMAPPED or have an weird big insert size (probably due to mismapping or bigger event)
*
* @param read the read to test
* @param basePos base position in REFERENCE coordinates (not read coordinates)
* @return whether or not the base is in the adaptor
*/
public static boolean isBaseInsideAdaptor(final GATKSAMRecord read, long basePos) {
final int adaptorBoundary = read.getAdaptorBoundary();
if (adaptorBoundary == CANNOT_COMPUTE_ADAPTOR_BOUNDARY || read.getInferredInsertSize() > DEFAULT_ADAPTOR_SIZE)
return false;
return read.getReadNegativeStrandFlag() ? basePos <= adaptorBoundary : basePos >= adaptorBoundary;
}
代码示例来源:origin: broadgsa/gatk
private static String insertLengthOutput(final ReadBackedPileup pileup) {
Integer[] insertSizes=new Integer[pileup.depthOfCoverage()];
int i=0;
for ( PileupElement p : pileup ) {
insertSizes[i]=p.getRead().getInferredInsertSize();
++i;
}
return Utils.join(",",insertSizes);
}
代码示例来源:origin: broadgsa/gatk
@Override
public Integer map(ReferenceContext referenceContext, GATKSAMRecord read, RefMetaDataTracker RefMetaDataTracker) {
final String rgID = read.getReadGroup().getId();
final PerReadGroupInfo info = readGroupInfo.get(rgID);
if ( info.needsMoreData() ) {
info.readLength.add(read.getReadLength());
info.nReadsSeen++;
if ( read.getReadPairedFlag() ) {
info.nReadsPaired++;
if ( read.getInferredInsertSize() != 0) {
info.insertSize.add(Math.abs(read.getInferredInsertSize()));
}
}
}
return null;
}
代码示例来源:origin: broadgsa/gatk
@Test
public void testSecondaryAlignmentsDoNotInterfere() {
final List<GATKSAMRecord> properReads = ArtificialSAMUtils.createPair(header, "foo", 1, 10, 30, true, false);
final GATKSAMRecord read1 = properReads.get(0);
read1.setAlignmentStart(8); // move the read
read1.setFlags(99); // first in proper pair, mate negative strand
final GATKSAMRecord read2Primary = properReads.get(1);
read2Primary.setFlags(147); // second in pair, mate unmapped, not primary alignment
Assert.assertEquals(read1.getInferredInsertSize(), 21);
final GATKSAMRecord read2NonPrimary = new GATKSAMRecord(read2Primary);
read2NonPrimary.setFlags(393); // second in proper pair, on reverse strand
final ConstrainedMateFixingManager manager = new ConstrainedMateFixingManager(null, genomeLocParser, 1000, 1000, 1000);
manager.addRead(read1, true, false);
manager.addRead(read2NonPrimary, false, false);
manager.addRead(read2Primary, false, false);
Assert.assertEquals(manager.getNReadsInQueue(), 3);
for ( final SAMRecord read : manager.getReadsInQueueForTesting() ) {
if ( read.getFirstOfPairFlag() ) {
Assert.assertEquals(read.getFlags(), 99);
Assert.assertEquals(read.getInferredInsertSize(), 23);
} else if ( read.getNotPrimaryAlignmentFlag() ) {
Assert.assertEquals(read.getFlags(), 393);
Assert.assertEquals(read.getInferredInsertSize(), -21);
} else {
Assert.assertEquals(read.getFlags(), 147);
Assert.assertEquals(read.getInferredInsertSize(), -23);
}
}
}
代码示例来源:origin: broadgsa/gatk-protected
/**
* Creates a hard-clipped view on a existing read record.
* @param read the underlying unclipped read.
* @param start inclusive first position in {@code read} included in the clipped view.
* @param end inclusive last position in {@code read} included in the clipped view.
*/
public ClippedGATKSAMRecord(final GATKSAMRecord read, int start, int end) {
super(read.getHeader());
this.setReferenceIndex(read.getReferenceIndex());
this.setAlignmentStart(read.getAlignmentStart() + start);
this.setMappingQuality(100);
// setting read indexing bin below
this.setFlags(read.getFlags());
this.setMateReferenceIndex(read.getMateReferenceIndex());
this.setMateAlignmentStart(read.getMateAlignmentStart());
this.setInferredInsertSize(read.getInferredInsertSize());
this.setReadBases(Arrays.copyOfRange(read.getReadBases(), start, end));
this.setBaseQualities(Arrays.copyOfRange(read.getBaseQualities(),start,end));
this.setReadName(read.getReadName());
insertionQuals = Arrays.copyOfRange(read.getBaseInsertionQualities(),start,end);
deletionQuals = Arrays.copyOfRange(read.getBaseDeletionQualities(),start,end);
// Set these to null in order to mark them as being candidates for lazy initialization.
// If this is not done, they will have non-null defaults.
super.setReadName(null);
super.setCigarString(null);
super.setReadBases(null);
super.setBaseQualities(null);
// Do this after the above because setCigarString will clear it.
GATKBin.setReadIndexingBin(this, -1);
}
代码示例来源:origin: broadgsa/gatk
emptyRead.setMateReferenceIndex(read.getMateReferenceIndex());
emptyRead.setMateAlignmentStart(read.getMateAlignmentStart());
emptyRead.setInferredInsertSize(read.getInferredInsertSize());
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